02 Bacteria Culture
- Blood: enriched (blood) and differential (blood pattern)
- Eosin methylene blue: selective (gm-) and differential (lactose fermenting)
Fastidious bacteria
Blood Agar
Chocolate Agar
.
- staph aureus beta hemolysis first
VPN
Aka thayer-martin media.
.
Bordet-Gengou agar
Aka potato agar.
.
- made from potatoes - high in starch
- selective for C. dphtheria.
Tellurite plate
- differential for c. diphtheria. Reduces tellurite to tellurium
- Gray black colonies.
Lowenstein-Jensen
Eaton's agar
- culture for mycoplasma pneumonia.
MacConkey's agar
.
Eosin-Methylene Blue
.
Sorbitol MacConkey
.
.
- charcoal for legionella
- classic: nursing home with atypical pneumonia
Sabouraud's agar
.
Special growth requirements
Superoxide dismutase and catalase
- O2 radical toxic to bacteria. Must be neutralized by aerobic organisms
Aerobes
Anaerobes
- bacteroides: appendicitis
- Aspirate vomit
- Clindamycin for anaerobe above diaphragm (lung)
- Metronidazole for anaerobe below diaphragm
- Also can use cipro/quinolone for diverticulitis/appendicitis
Intracellular
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